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rabbit da1e mab igg xp isotype control  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit da1e mab igg xp isotype control
    Rabbit Da1e Mab Igg Xp Isotype Control, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1278 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+control+igg/Rabbit+mAb+IgG+XP+Isotype+Control/pmc13010434-12-0-9
    Average 97 stars, based on 1278 article reviews
    rabbit da1e mab igg xp isotype control - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Control:

    Article Title: Dihydroartemisinin inhibits NSCLC by targeting MDM2/c-Myc axis independent of p53.
    Article Snippet: The antimalarial agent Dihydroartemisinin (DHA) has significant potential for drug repurposing for cancer therapy including non-small cell lung cancer (NSCLC).. However, its underlying anti-tumor mechanisms remain to be elucidated.. In this study, we demonstrated that DHA suppressed the mouse double minute 2 (MDM2) in A549 and H1299 cells, whereas MDM2 overexpression effectively restored DHA’s inhibition on both NSCLC cell lines.

    Article Title: USP5 Binds and Stabilizes EphA2 to Increase Nasopharyngeal Carcinoma Radioresistance
    Article Snippet: .. Antibodies used in this study included USP5 (sc-390943, Santa Cruz), USP5 (A4202, Abclonal), EphA2 (sc-398832, Santa Cruz), GAPDH (AC002, Abclonal), Myc-tag (AE010, Abclonal), Flag-Tag (AE092, Abclonal), HA-Tag (66006-2-Ig, Proteintech), His-Tag (AE003, Abclonal), GST-Tag (SAB4301139, Sigma-Aldrich), Ubiquitin (91112, CST), γH2AX (2577, CST), cleaved caspase 3 (9661, CST), Ki67 (sc-23900, Santa Cruz), HRP anti-rabbit IgG (7074, CST), HRP anti-mouse IgG (7076, CST), Rabbit control IgG (3900, CST), Mouse control IgG (5415, CST). .. Protein G/A-SepharoseTM 4B (82085), LipofectamineTM 2000 (11668019) and Trizol (15596026) were purchased from ThermoFischer Scientific.

    Article Title: Decreased expression of circ-CCDC134 mediated by TNF-α in patients with rheumatoid arthritis affects T cell function via targeting protein phosphatase 2A
    Article Snippet: .. Anti-PPP2R1A antibody or Rabbit Control IgG (Cell Signaling Technology, Danvers, MA, USA) were used as the antibodies for immunoprecipitation. .. The obtained RNA was isolated using the RNA Clean & Concentrator-5 kits (Zymo Research, Irvine, CA, USA) and analyzed using real-time RT-PCR.

    Article Title: Grhl3 coordinates epithelial gene activation and epigenetic remodeling in fibroblasts.
    Article Snippet: The mesenchymal-to-epithelial transition (MET) is a critical and stepwise process in cellular reprogramming and development.. Grhl3 has been implicated in MET, but its capacity to initiate epithelial programs in non-epithelial cells remains incompletely defined.. Here, we demonstrate that Grhl3 overexpression in MEFs induces a transcriptional shift consistent with early MET, characterized by activation of epithelial genes and suppression of mesenchymal features.

    Article Title: AKT activation triggers Rab14-mediated ADAM10 translocation to the cell surface in human aortic endothelial cells.
    Article Snippet: The cells were lysed with NP-40 Lysis Buffer (MedChemExpress), which contains 50 mM Tris (pH 7.4), 150 mM NaCl, 1% NP-40, and protease inhibitors. .. At 4 °C, the whole cell lysates (500 μg) were precleared by incubating with 1 μg of rabbit control IgG (Cell Signaling Technology, #2729) and 20 μl of protein A agarose (Santa Cruz Biotechnology, sc-2001) for 30 min and then incubated overnight with 4 μg of a rabbit anti-ADAM10 antibody (MA5-32616) or a rabbit control IgG, followed by incubation with 20 μl of protein A agarose for 4 h. Both the whole cell lysate (input) and the immunoprecipitated proteins were analyzed by Western blot using mouse monoclonal antibodies to ADAM10 (sc-28358) and Rab14 (sc-271401). .. Separation of cell surface and intracellular proteins The PierceTM cell surface protein biotinylation and isolation kit (ThermoFisher Scientific) was used to separate cell surface proteins from intracellular proteins.

    Article Title: AKT activation triggers Rab14-mediated ADAM10 translocation to the cell surface in human aortic endothelial cells
    Article Snippet: The cells were lysed with NP-40 Lysis Buffer (MedChemExpress), which contains 50 mM Tris (pH 7.4), 150 mM NaCl, 1% NP-40, and protease inhibitors. .. At 4 °C, the whole cell lysates (500 μg) were precleared by incubating with 1 μg of rabbit control IgG (Cell Signaling Technology, #2729) and 20 μl of protein A agarose (Santa Cruz Biotechnology, sc-2001) for 30 min and then incubated overnight with 4 μg of a rabbit anti-ADAM10 antibody (MA5-32616) or a rabbit control IgG, followed by incubation with 20 μl of protein A agarose for 4 h. Both the whole cell lysate (input) and the immunoprecipitated proteins were analyzed by Western blot using mouse monoclonal antibodies to ADAM10 (sc-28358) and Rab14 (sc-271401). .. The PierceTM cell surface protein biotinylation and isolation kit (ThermoFisher Scientific) was used to separate cell surface proteins from intracellular proteins.

    Article Title: Decreased expression of circ-CCDC134 mediated by TNF-α in patients with rheumatoid arthritis affects T cell function via targeting protein phosphatase 2A.
    Article Snippet: .. Anti-PPP2R1A antibody or Rabbit Control IgG (Cell Signaling Technology, Danvers, MA, USA) were used as the antibodies for immunoprecipitation. .. The obtained RNA was isolated using the RNA Clean & Concentrator-5 kits (Zymo Research, Irvine, CA, USA) and analyzed using real-time RT-PCR.

    Article Title: The deubiquitinase OTUD1 deubiquitinates TIPE2 and plays a protective role in sepsis-induced lung injury by targeting TAK1-mediated MAPK and NF-κB signaling.
    Article Snippet: Ovarian tumor domain-containing protease 1 (OTUD1) is a critical negative regulator that promotes innate immune homeostasis and is extensively involved in the pathogenesis of sepsis.. In this study, we performed a powerful integration of multiomics analysis and an experimental mechanistic investigation to elucidate the immunoregulatory role of OTUD1 in sepsis at the clinical, animal and cellular levels.. Our study revealed the upregulation of OTUD1 expression and the related distinctive alterations observed via multiomics profiling in clinical and experimental sepsis.

    Incubation:

    Article Title: Dihydroartemisinin inhibits NSCLC by targeting MDM2/c-Myc axis independent of p53.
    Article Snippet: The antimalarial agent Dihydroartemisinin (DHA) has significant potential for drug repurposing for cancer therapy including non-small cell lung cancer (NSCLC).. However, its underlying anti-tumor mechanisms remain to be elucidated.. In this study, we demonstrated that DHA suppressed the mouse double minute 2 (MDM2) in A549 and H1299 cells, whereas MDM2 overexpression effectively restored DHA’s inhibition on both NSCLC cell lines.

    Article Title: Grhl3 coordinates epithelial gene activation and epigenetic remodeling in fibroblasts.
    Article Snippet: The mesenchymal-to-epithelial transition (MET) is a critical and stepwise process in cellular reprogramming and development.. Grhl3 has been implicated in MET, but its capacity to initiate epithelial programs in non-epithelial cells remains incompletely defined.. Here, we demonstrate that Grhl3 overexpression in MEFs induces a transcriptional shift consistent with early MET, characterized by activation of epithelial genes and suppression of mesenchymal features.

    Article Title: AKT activation triggers Rab14-mediated ADAM10 translocation to the cell surface in human aortic endothelial cells.
    Article Snippet: The cells were lysed with NP-40 Lysis Buffer (MedChemExpress), which contains 50 mM Tris (pH 7.4), 150 mM NaCl, 1% NP-40, and protease inhibitors. .. At 4 °C, the whole cell lysates (500 μg) were precleared by incubating with 1 μg of rabbit control IgG (Cell Signaling Technology, #2729) and 20 μl of protein A agarose (Santa Cruz Biotechnology, sc-2001) for 30 min and then incubated overnight with 4 μg of a rabbit anti-ADAM10 antibody (MA5-32616) or a rabbit control IgG, followed by incubation with 20 μl of protein A agarose for 4 h. Both the whole cell lysate (input) and the immunoprecipitated proteins were analyzed by Western blot using mouse monoclonal antibodies to ADAM10 (sc-28358) and Rab14 (sc-271401). .. Separation of cell surface and intracellular proteins The PierceTM cell surface protein biotinylation and isolation kit (ThermoFisher Scientific) was used to separate cell surface proteins from intracellular proteins.

    Article Title: AKT activation triggers Rab14-mediated ADAM10 translocation to the cell surface in human aortic endothelial cells
    Article Snippet: The cells were lysed with NP-40 Lysis Buffer (MedChemExpress), which contains 50 mM Tris (pH 7.4), 150 mM NaCl, 1% NP-40, and protease inhibitors. .. At 4 °C, the whole cell lysates (500 μg) were precleared by incubating with 1 μg of rabbit control IgG (Cell Signaling Technology, #2729) and 20 μl of protein A agarose (Santa Cruz Biotechnology, sc-2001) for 30 min and then incubated overnight with 4 μg of a rabbit anti-ADAM10 antibody (MA5-32616) or a rabbit control IgG, followed by incubation with 20 μl of protein A agarose for 4 h. Both the whole cell lysate (input) and the immunoprecipitated proteins were analyzed by Western blot using mouse monoclonal antibodies to ADAM10 (sc-28358) and Rab14 (sc-271401). .. The PierceTM cell surface protein biotinylation and isolation kit (ThermoFisher Scientific) was used to separate cell surface proteins from intracellular proteins.

    Ubiquitin Proteomics:

    Article Title: USP5 Binds and Stabilizes EphA2 to Increase Nasopharyngeal Carcinoma Radioresistance
    Article Snippet: .. Antibodies used in this study included USP5 (sc-390943, Santa Cruz), USP5 (A4202, Abclonal), EphA2 (sc-398832, Santa Cruz), GAPDH (AC002, Abclonal), Myc-tag (AE010, Abclonal), Flag-Tag (AE092, Abclonal), HA-Tag (66006-2-Ig, Proteintech), His-Tag (AE003, Abclonal), GST-Tag (SAB4301139, Sigma-Aldrich), Ubiquitin (91112, CST), γH2AX (2577, CST), cleaved caspase 3 (9661, CST), Ki67 (sc-23900, Santa Cruz), HRP anti-rabbit IgG (7074, CST), HRP anti-mouse IgG (7076, CST), Rabbit control IgG (3900, CST), Mouse control IgG (5415, CST). .. Protein G/A-SepharoseTM 4B (82085), LipofectamineTM 2000 (11668019) and Trizol (15596026) were purchased from ThermoFischer Scientific.

    Immunoprecipitation:

    Article Title: Decreased expression of circ-CCDC134 mediated by TNF-α in patients with rheumatoid arthritis affects T cell function via targeting protein phosphatase 2A
    Article Snippet: .. Anti-PPP2R1A antibody or Rabbit Control IgG (Cell Signaling Technology, Danvers, MA, USA) were used as the antibodies for immunoprecipitation. .. The obtained RNA was isolated using the RNA Clean & Concentrator-5 kits (Zymo Research, Irvine, CA, USA) and analyzed using real-time RT-PCR.

    Article Title: AKT activation triggers Rab14-mediated ADAM10 translocation to the cell surface in human aortic endothelial cells.
    Article Snippet: The cells were lysed with NP-40 Lysis Buffer (MedChemExpress), which contains 50 mM Tris (pH 7.4), 150 mM NaCl, 1% NP-40, and protease inhibitors. .. At 4 °C, the whole cell lysates (500 μg) were precleared by incubating with 1 μg of rabbit control IgG (Cell Signaling Technology, #2729) and 20 μl of protein A agarose (Santa Cruz Biotechnology, sc-2001) for 30 min and then incubated overnight with 4 μg of a rabbit anti-ADAM10 antibody (MA5-32616) or a rabbit control IgG, followed by incubation with 20 μl of protein A agarose for 4 h. Both the whole cell lysate (input) and the immunoprecipitated proteins were analyzed by Western blot using mouse monoclonal antibodies to ADAM10 (sc-28358) and Rab14 (sc-271401). .. Separation of cell surface and intracellular proteins The PierceTM cell surface protein biotinylation and isolation kit (ThermoFisher Scientific) was used to separate cell surface proteins from intracellular proteins.

    Article Title: AKT activation triggers Rab14-mediated ADAM10 translocation to the cell surface in human aortic endothelial cells
    Article Snippet: The cells were lysed with NP-40 Lysis Buffer (MedChemExpress), which contains 50 mM Tris (pH 7.4), 150 mM NaCl, 1% NP-40, and protease inhibitors. .. At 4 °C, the whole cell lysates (500 μg) were precleared by incubating with 1 μg of rabbit control IgG (Cell Signaling Technology, #2729) and 20 μl of protein A agarose (Santa Cruz Biotechnology, sc-2001) for 30 min and then incubated overnight with 4 μg of a rabbit anti-ADAM10 antibody (MA5-32616) or a rabbit control IgG, followed by incubation with 20 μl of protein A agarose for 4 h. Both the whole cell lysate (input) and the immunoprecipitated proteins were analyzed by Western blot using mouse monoclonal antibodies to ADAM10 (sc-28358) and Rab14 (sc-271401). .. The PierceTM cell surface protein biotinylation and isolation kit (ThermoFisher Scientific) was used to separate cell surface proteins from intracellular proteins.

    Article Title: Decreased expression of circ-CCDC134 mediated by TNF-α in patients with rheumatoid arthritis affects T cell function via targeting protein phosphatase 2A.
    Article Snippet: .. Anti-PPP2R1A antibody or Rabbit Control IgG (Cell Signaling Technology, Danvers, MA, USA) were used as the antibodies for immunoprecipitation. .. The obtained RNA was isolated using the RNA Clean & Concentrator-5 kits (Zymo Research, Irvine, CA, USA) and analyzed using real-time RT-PCR.

    Sonication:

    Article Title: Grhl3 coordinates epithelial gene activation and epigenetic remodeling in fibroblasts.
    Article Snippet: The mesenchymal-to-epithelial transition (MET) is a critical and stepwise process in cellular reprogramming and development.. Grhl3 has been implicated in MET, but its capacity to initiate epithelial programs in non-epithelial cells remains incompletely defined.. Here, we demonstrate that Grhl3 overexpression in MEFs induces a transcriptional shift consistent with early MET, characterized by activation of epithelial genes and suppression of mesenchymal features.

    Chromatin Immunoprecipitation:

    Article Title: Grhl3 coordinates epithelial gene activation and epigenetic remodeling in fibroblasts.
    Article Snippet: The mesenchymal-to-epithelial transition (MET) is a critical and stepwise process in cellular reprogramming and development.. Grhl3 has been implicated in MET, but its capacity to initiate epithelial programs in non-epithelial cells remains incompletely defined.. Here, we demonstrate that Grhl3 overexpression in MEFs induces a transcriptional shift consistent with early MET, characterized by activation of epithelial genes and suppression of mesenchymal features.

    Western Blot:

    Article Title: AKT activation triggers Rab14-mediated ADAM10 translocation to the cell surface in human aortic endothelial cells.
    Article Snippet: The cells were lysed with NP-40 Lysis Buffer (MedChemExpress), which contains 50 mM Tris (pH 7.4), 150 mM NaCl, 1% NP-40, and protease inhibitors. .. At 4 °C, the whole cell lysates (500 μg) were precleared by incubating with 1 μg of rabbit control IgG (Cell Signaling Technology, #2729) and 20 μl of protein A agarose (Santa Cruz Biotechnology, sc-2001) for 30 min and then incubated overnight with 4 μg of a rabbit anti-ADAM10 antibody (MA5-32616) or a rabbit control IgG, followed by incubation with 20 μl of protein A agarose for 4 h. Both the whole cell lysate (input) and the immunoprecipitated proteins were analyzed by Western blot using mouse monoclonal antibodies to ADAM10 (sc-28358) and Rab14 (sc-271401). .. Separation of cell surface and intracellular proteins The PierceTM cell surface protein biotinylation and isolation kit (ThermoFisher Scientific) was used to separate cell surface proteins from intracellular proteins.

    Article Title: AKT activation triggers Rab14-mediated ADAM10 translocation to the cell surface in human aortic endothelial cells
    Article Snippet: The cells were lysed with NP-40 Lysis Buffer (MedChemExpress), which contains 50 mM Tris (pH 7.4), 150 mM NaCl, 1% NP-40, and protease inhibitors. .. At 4 °C, the whole cell lysates (500 μg) were precleared by incubating with 1 μg of rabbit control IgG (Cell Signaling Technology, #2729) and 20 μl of protein A agarose (Santa Cruz Biotechnology, sc-2001) for 30 min and then incubated overnight with 4 μg of a rabbit anti-ADAM10 antibody (MA5-32616) or a rabbit control IgG, followed by incubation with 20 μl of protein A agarose for 4 h. Both the whole cell lysate (input) and the immunoprecipitated proteins were analyzed by Western blot using mouse monoclonal antibodies to ADAM10 (sc-28358) and Rab14 (sc-271401). .. The PierceTM cell surface protein biotinylation and isolation kit (ThermoFisher Scientific) was used to separate cell surface proteins from intracellular proteins.

    Bioprocessing:

    Article Title: AKT activation triggers Rab14-mediated ADAM10 translocation to the cell surface in human aortic endothelial cells.
    Article Snippet: The cells were lysed with NP-40 Lysis Buffer (MedChemExpress), which contains 50 mM Tris (pH 7.4), 150 mM NaCl, 1% NP-40, and protease inhibitors. .. At 4 °C, the whole cell lysates (500 μg) were precleared by incubating with 1 μg of rabbit control IgG (Cell Signaling Technology, #2729) and 20 μl of protein A agarose (Santa Cruz Biotechnology, sc-2001) for 30 min and then incubated overnight with 4 μg of a rabbit anti-ADAM10 antibody (MA5-32616) or a rabbit control IgG, followed by incubation with 20 μl of protein A agarose for 4 h. Both the whole cell lysate (input) and the immunoprecipitated proteins were analyzed by Western blot using mouse monoclonal antibodies to ADAM10 (sc-28358) and Rab14 (sc-271401). .. Separation of cell surface and intracellular proteins The PierceTM cell surface protein biotinylation and isolation kit (ThermoFisher Scientific) was used to separate cell surface proteins from intracellular proteins.

    Article Title: AKT activation triggers Rab14-mediated ADAM10 translocation to the cell surface in human aortic endothelial cells
    Article Snippet: The cells were lysed with NP-40 Lysis Buffer (MedChemExpress), which contains 50 mM Tris (pH 7.4), 150 mM NaCl, 1% NP-40, and protease inhibitors. .. At 4 °C, the whole cell lysates (500 μg) were precleared by incubating with 1 μg of rabbit control IgG (Cell Signaling Technology, #2729) and 20 μl of protein A agarose (Santa Cruz Biotechnology, sc-2001) for 30 min and then incubated overnight with 4 μg of a rabbit anti-ADAM10 antibody (MA5-32616) or a rabbit control IgG, followed by incubation with 20 μl of protein A agarose for 4 h. Both the whole cell lysate (input) and the immunoprecipitated proteins were analyzed by Western blot using mouse monoclonal antibodies to ADAM10 (sc-28358) and Rab14 (sc-271401). .. The PierceTM cell surface protein biotinylation and isolation kit (ThermoFisher Scientific) was used to separate cell surface proteins from intracellular proteins.



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